Recombinant SARS CoV-2 RBD

Details

Catalog number: RF0119

Format: 50ug, 1 mg

Origin: It is produced by transient expression in non-transgenic plants. Recombinant SARS CoV-2 RBD is purified by affinity chromatography. This product contains no animal–derived components or impurities. Animal Free product.

Description: Recombinant SARS-CoV-2 RBD protein consists of 97 amino acids fused to a 6 His tag at C-terminal. It has a predicted molecular mass of 11,1 kDa. The Spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the beginning of the infection process. It´s been reported that 2019-nCoV can infect the human respiratory epithelial cells through the interaction with the human ACE2 receptor. The Spike protein is a transmembrane protein containing two subunits, S1 and S2. S1 contain a receptor binding domain (RBD) which is responsible for recognizing the cell receptor. The S protein play a role key in the induction of neutralizing-antibody and T-cell responses. And, the receptor binding -domain (RBD) represents a promising antigen for detecting CoV-2 specific antibodies in potential affected individuals.

Species: SARS-CoV-2

Purity: > 95% by SDS-PAGE gel

Endotoxin: < 0.04 EU / μg protein (LAL method)

Formulation: Lyophilized TrisHCl buffer pH 8.5. Sterile filtered through 0.2 µm filter.

Reconstitution: Always centrifuge the vial before opening. Lyophilized protein should be reconstituted in water following instructions of batch Quality Control Sheet. At higher concentrations solubility may be reduced and, due to the protein nature, dimmers and multimers generated. Optimal concentration should be determined for specific application.

Storage and Stability: This lyophilized preparation is stable at 2-8º C for short term. For longer storage it should be kept at -20ºC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.