Recombinant Human TIMP-2

Details

Catalog number: RF0111

Format: 50ug, 1 mg

Origin: It is produced by transient expression in non-transgenic plants. Recombinant Human TIMP-2 is purified by sequential chromatography (FPLC). This product contains no animal–derived components or impurities. Animal Free product.

Description: Recombinant human TIMP-2 is a 22.5 kDa protein containing 194 amino acid residues fused to a 6 His tag at N-terminal. TIMPs are naturally-occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tight bound inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. MMPs play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis, and tissue remodeling.

Species: Human

Purity: > 95% by SDS-PAGE gel

Endotoxin: < 0.04 EU / μg protein (LAL method)

Formulation: Lyophilized PBS buffer pH 6,8-7,5. Sterile filtered through 0.2 µm filter.

Reconstitution: Always centrifuge the vial before opening. Lyophilized protein should be reconstituted in water following instructions of batch Quality Control Sheet. At higher concentrations solubility may be reduced and, due to the protein nature, dimmers and multimers generated. Optimal concentration should be determined for specific application.

Storage and Stability: This lyophilized preparation is stable at 2-8º C for short term. For longer storage it should be kept at -20ºC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.