Recombinant Human IL10

Details

Catalog number: RF0103

Format: 50ug, 1 mg

Origin: It is produced by transient expression in non-transgenic plants. Recombinant human IL-10 is purified by by standard protein purification methods. This product contains no animal–derived components or impurities. Animal Free product.

Description: Recombinant human IL-10 is a glycosylated, non-disulfide linked homodimer with an apparent molecular mass of 17 kDa due to glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Interleukin 10 is an anti-inflammatory cytokine initially characterized as a T helper (TH)2 specific cytokine; however, further investigations revealed that IL- 10 production was also associated with T regulatory cell responses. IL-10 works by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including, IFN-γ, TNF-α, IL-2, and IL-3, IL-4, and GM-CSF.

Species: Human

Purity: > 95% by SDS-PAGE gel

Endotoxin: < 0.04 EU / μg protein (LAL method)

Formulation: Lyophilized TrisHCl buffer pH 7.4. Sterile filtered through 0.2 µm filter.

Reconstitution: Always centrifuge the vial before opening. Lyophilized protein should be reconstituted in water following instructions of batch Quality Control Sheet. At higher concentrations solubility may be reduced and, due to the protein nature, dimmers and multimers generated. Optimal concentration should be determined for specific application.

Storage and Stability: This lyophilized preparation is stable at 2-8º C for short term. For longer storage it should be kept at -20ºC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.