Recombinant Human IGF1

Details

Catalog number: RF0107

Format: 50ug, 1 mg

Origin: It is produced by transient expression in non-transgenic plants. Recombinant human IGF1 is purified by sequential chromatography methods (FPLC). This product contains no animal–derived components or impurities. Animal Free product.

Description: Recombinant human IGF1 is 8.7 kDa polypeptide chain containing 70 amino acids fused to a 8 His tag at N-terminal. IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. IGF-1 shares a high degree of structural and functional homology with the insulin hormone. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis. It is a mitogenic agent for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. It also plays an important role in some differentiation and anti-apoptotic functions, so it has a key role in tissue renewal and repair. In tissues, IGF-I is produced by mesenchymal type cells and acts in a paracrine fashion and /or an autocrine fashion by binding to the IGF-IR. This binding activates the receptor tyrosine kinase that triggers the downstream responses and finally stimulates cell division. His potent mitogenic activity is determinate by the availability of free IGF-1 to interact with IGF-1 receptors. Insulin and the IGFs elicit the same biological responses, either by cross-reacting with one of the receptors for the heterologous ligand or by concurrent activation of convergent effector pathways by binding to the homologous receptor.

Species: Human

Purity: > 95% by SDS-PAGE gel

Endotoxin: < 0.04 EU / μg protein (LAL method)

Formulation: Lyophilized TrisHCl buffer pH 7.4. Sterile filtered through 0.2 µm filter.

Reconstitution: Always centrifuge the vial before opening. Lyophilized protein should be reconstituted in water following instructions of batch Quality Control Sheet. At higher concentrations solubility may be reduced and, due to the protein nature, dimmers and multimers generated. Optimal concentration should be determined for specific application.

Storage and Stability: This lyophilized preparation is stable at 2-8º C for short term. For longer storage it should be kept at -20ºC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.