Recombinant Human GM-CSF

Details

Catalog number: RF0036

Format: 50ug, 1 mg

Origin: It is produced by transient expression in non-transgenic plants. Recombinant Human GM-CSF is purified by sequential chromatography FPLC). This product contains no animal–derived components or impurities. Animal Free product.

Description: Recombinant Human GM-CSF is a glycosilated polypeptide chain containing 127 aminoacids, fused to 10 His tag at N-terminal. It migrates as a broad band between 15 and 25kDa due to post-translational modifications, in particular glycosilation. It is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, such as granulocytes, macrophages, eosinophils and erythrocytes. Is involved in differentiation of dendritic cells and is a key factor in differentiation pathways leading form stem cells. GMCSF is produced by several cell types as monocytes, fibroblasts, endothelial cells and T- Lymphocytes in response to a number of inflammatory mediators present in the hemopoietic environment and peripheral site of inflammation. GMCSR has been identified to mediate the activation of Jak-Stat and MAPK pathways.

Species: Human

Purity: > 95% by SDS-PAGE gel

Endotoxin: < 0.04 EU / μg protein (LAL method)

Formulation: Lyophilized PBS pH 7.6 . Sterile filtered through 0.2 µm filter.

Reconstitution: Always centrifuge the vial before opening. Lyophilized protein should be reconstituted in water following instructions of batch Quality Control Sheet. At higher concentrations solubility may be reduced. Optimal concentration should be determined for specific application.

Storage and Stability: This lyophilized preparation is stable at 2-8º C for short term. For longer storage it should be kept at -20ºC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.