Human Recombinant ProteinsRF0113
Recombinant Human Collagen I
Catalog number: RF0113
Format: 50ug, 1 mg
Origin: It is produced by transient expression in non-transgenic plants. Recombinant human Collagen I is purified by sequential chromatography methods (FPLC). This product contains no animal–derived components or impurities. Animal Free product.
Description: Recombinant human Collagen I is a 10.7 kDa protein containing 116 amino acid residues fused to a 6 His tag N-terminal. rHuman Collagen migrates as a 14-15 kDa band under reducing conditions. Recombinant human Collagen I is a fragment of Collagen alpha-1(I) chain, the major structural component of skin, bone, tendon and other fibrous connective tissue. Type I Collagen is a key factor of the tensile properties of the ECM. The amino acid sequence is mainly a repeating motif with glycine in very third position with proline or 4-hydroxyproline preceding the glycine residue. Since this sequence contains the tripeptide Arg-Gly-Asp (RGD), considered as best know collagen cell surface receptors, recombinant collagen I could participate in the regulation and cellular signaling via the RGD motif.
Purity: > 95% by SDS-PAGE gel
Endotoxin: < 0.04 EU / μg protein (LAL method)
Formulation: Lyophilized Sodium Acetate Buffer pH 5-5,2 . Sterile filtered through 0.2 µm filter.
Reconstitution: Always centrifuge the vial before opening. Lyophilized protein should be reconstituted in water following instructions of batch Quality Control Sheet. At higher concentrations solubility may be reduced and, due to the protein nature, dimmers and multimers generated. Optimal concentration should be determined for specific application.
Storage and Stability: This lyophilized preparation is stable at 2-8º C for short term. For longer storage it should be kept at -20ºC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.