Recombinant Human Follistatin

Details

Catalog number: RF0043

Format: 50ug, 1 mg

Origin: It is produced by transient expression in non-transgenic plants. Recombinant human Follistatin is purified by sequential chromatography methods (FPLC). This product contains no animal–derived components or impurities. Animal Free product.

Description: Recombinant human Follistatin isoform 2 is a polypeptide chain containing 217 amino acids fused to a 6 His tag at N-terminal. It has a predicted molecular mass of 32.3 kDa. Follistatin is a monomeric glycoprotein that binds to ligands of the TGF-β superfamily and regulates their activity by inhibiting their access to signalling receptors. Follistatin received its name because it suppresses synthesis and secretion of follicle-stimulating hormone (FSH) from the pituitary gland.

Species: Human

Purity: > 95% by SDS-PAGE gel

Endotoxin: < 0.04 EU / μg protein (LAL method)

Formulation: Lyophilized TrisHCl buffer pH 7.4. Sterile filtered through 0.2 µm filter.

Reconstitution: Always centrifuge the vial before opening. Lyophilized protein should be reconstituted in water following instructions of batch Quality Control Sheet. At higher concentrations solubility may be reduced and, due to the protein nature, dimmers and multimers generated. Optimal concentration should be determined for specific application.

Storage and Stability: This lyophilized preparation is stable at 2-8º C for short term. For longer storage it should be kept at -20ºC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended.